For the rotor gene q and other rotor gene cyclers we recommend using the rotor gene sybr green pcr kit which has been specially developed for fast cycling on these. Applied biosystems fast sybr green master mix designed for speed provides a fast reliable and cost effective solution for rt pcr application without compromising sensitivity specificity dynamic range or pcr efficiency. Applied biosystems instruments assays and reagents have been trusted in the lab for over 20 years.
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